Anti-RhoA (phospho S188)抗体(ab41435)

概述

  • 产品名称Anti-RhoA (phospho S188)抗体
    参阅全部 RhoA 一抗
  • 描述
    兔多克隆抗体to RhoA (phospho S188)
  • 特异性Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide phosphorylated at Ser188, corresponding to amino acid residues around serine 188 of human RhoA, conjugated to KLH.

  • 阳性对照
    • Recombinant human RhoA GST fusion protein, phosphorylated with PKA.
  • 常规说明Do not aliquot.


    Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity.

性能

应用

Our Abpromise guarantee covers the use of ab41435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/2000.
WB 1/1000. Predicted molecular weight: 22 kDa.

靶标

  • 功能Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
  • 序列相似性Belongs to the small GTPase superfamily. Rho family.
  • 结构域The basic-rich region is essential for yopT recognition and cleavage.
  • 翻译后修饰Substrate for botulinum ADP-ribosyltransferase.
    Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
    AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
    Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • 细胞定位Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aplysia ras related homolog 12 antibody
    • ARH12 antibody
    • ARHA antibody
    • H 12 antibody
    • H12 antibody
    • Oncogene RHO H12 antibody
    • Ras homolog family member A antibody
    • Ras homolog gene family member A antibody
    • Rho A antibody
    • Rho cDNA clone 12 antibody
    • RHO H12 antibody
    • RHO12 antibody
    • RHOA antibody
    • RHOA_HUMAN antibody
    • RHOH12 antibody
    • Small GTP binding protein Rho A antibody
    • Transforming protein Rho A antibody
    • Transforming protein RhoA antibody
    see all

Anti-RhoA (phospho S188) antibody 图像

  • Lanes 1 - 2 : anti-Rho
    Lanes 3 - 6 : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution

    Lane 1 : recombinant human RhoA GST fusion protein, unphosphorylated
    Lane 2 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
    Lane 3 : recombinant human RhoA GST fusion protein, unphosphorylated
    Lane 4 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
    Lane 5 : recombinant human RhoA GST fusion protein, phosphorylated with PKA with RhoA (phospho S188) peptide
    Lane 6 : recombinant human RhoA GST fusion protein, phosphorylated with PKA with non-specific phosphoserine peptide


    Predicted band size : 22 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

Anti-RhoA (phospho S188) antibody (ab41435)参考文献

This product has been referenced in:
  • Li Y  et al. Small GTPases Rap1 and RhoA regulate superoxide formation by Rac1 GTPases activation during the phagocytosis of IgG-opsonized zymosans in macrophages. Free Radic Biol Med 52:1796-1805 (2012). WB ; Mouse . Read more (PubMed: 22330068) »
  • Lawrence BD  et al. Silk film topography directs collective epithelial cell migration. PLoS One 7:e50190 (2012). WB, ICC/IF ; Human . Read more (PubMed: 23185573) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - nuclear (liver)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 14 2015

Application Western blot
Sample Pig Cell lysate - whole cell (Swine testicle cells)
Loading amount 20 µg
Specification Swine testicle cells
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Thary Jacob

Verified customer

提交于 Jan 24 2013

DISCOUNT CODE: ABTRIAL-EBXXX

Expiration date: 17/03/2013
Value: £227.00

I am very pleased to hear you would like to accept our offer and test ab41435 in Pig. This code will give you £227.00 off your next order befo...

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Thank you for your interest.

As it has been described in the previous e-mail the discount code for the value of the product and as soon as the Abreview is submitted, the discount code will become active.

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Thank you very much for your interest our AbTrial program.


To our knowledge,ab41435 has not been tested in Pig. However by participating in our AbTrial program you can now use our products in an untested application or species without f...

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We have observed both the 27 kDa and the 70 kDa band using RP1361. We have not identified the 70 kDa band, and have no evidence that it is related to RhoA. We have shown that antibody does detect RhoA at the lower molecular weight, as well as detects r...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 30 µg
Specification HeLa Cells
Gel Running Conditions Non-reduced Denaturing (12.5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 08 2012

I am very pleased to hear you would like to accept our offer and test ab41435 in mouse in ICC/IF. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse in ICC/IF and include ...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for ...

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