兔多克隆抗体to Rho guanine exchange factor 16
Chimpanzee, Gorilla, Orangutan
Synthetic peptide designed within amino acids 125 - 175 of Human Rho guanine exchange factor 16 (NP_055263.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. Contains 1 SH3 domain.
The PDZ-binding motif mediates interaction with TAX1BP3.
Information by UniProt
ARHGEF 16 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16 antibody (ab86068)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Rho guanine exchange factor 16 with ab86068 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Rho guanine exchange factor 16 antibody (ab86068)
All lanes : Anti-Rho guanine exchange factor 16 antibody (ab86068) at 0.04 µg/ml Lane 1 : Hela whole cell lysate at 50 µg Lane 2 : Hela whole cell lysate at 15 µg Lane 3 : Hela whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 80 kDa Exposure time : 30 seconds
Immunoprecipitation - Rho guanine exchange factor 16 antibody (ab86068)
Detection of Rho guanine exchange factor 16 by Western blot of Immunprecipitate.
ab86068, at 1 µg/ml, staining Rho guanine exchange factor 16 in HeLa whole cell lysate immunoprecipitated using ab86068 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"