1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
Detection of Rho guanine exchange factor 16 by Western blot of Immunprecipitate.
ab86068, at 1 µg/ml, staining Rho guanine exchange factor 16 in HeLa whole cell lysate immunoprecipitated using ab86068 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.