概述

  • 产品名称Anti-Rho抗体[Y486]
    参阅全部 Rho 一抗
  • 描述
    兔单克隆抗体[Y486] to Rho
  • 特异性The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the N-term of human Rho.

  • 阳性对照
    • PC12 cell lysate and hepatocellular carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Rho antibody (Alexa Fluor® 488) [Y486] (ab203324)

    Anti-Rho antibody (Alexa Fluor® 647) [Y486] (ab203325)

性能

应用

Our Abpromise guarantee covers the use of ab32046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    • 序列相似性Belongs to the small GTPase superfamily. Rho family.
    • 结构域The basic-rich region is essential for yopT recognition and cleavage.
    • 翻译后修饰Substrate for botulinum ADP-ribosyltransferase.
      Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
      AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
      Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
      Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
    • 细胞定位Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Cytoplasm > cell cortex. Midbody. Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARH12 antibody
      • ARHA antibody
      • H12 antibody
      • ras homolog gene family member A antibody
      • ras homolog gene family member B antibody
      • ras homolog gene family member C antibody
      • Rho cDNA clone 12 antibody
      • RHO12 antibody
      • RHOA antibody
      • RHOA_HUMAN antibody
      • rhob antibody
      • rhoc antibody
      • RHOH12 antibody
      • Small GTP binding protein RhoA antibody
      • Transforming protein RhoA antibody
      see all

    Anti-Rho antibody [Y486] 图像

    • Anti-Rho antibody [Y486] (ab32046) at 1/2000 dilution + PC12 cell lysate

      Predicted band size : 21 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab32046 at 1/100 dilution.

    Anti-Rho antibody [Y486] (ab32046)参考文献

    This product has been referenced in:
    • Ruggeri RM  et al. The tyrosine kinase receptor c-met, its cognate ligand HGF and the tyrosine kinase receptor trasducers STAT3, PI3K and RHO in thyroid nodules associated with Hashimoto's thyroiditis: an immunohistochemical characterization. Eur J Histochem 54:e24 (2010). Read more (PubMed: 20558345) »
    • Laumanns IP  et al. The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension. Am J Respir Cell Mol Biol 40:683-91 (2009). Read more (PubMed: 19029018) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (lung epithelial cells)
    Specification lung epithelial cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% saponin
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 31 2012

    Thank you for your enquiry. I have been in touch with the lab for their comments. Unfortunately this has not been tested. From a literature search I can see that there are 3 binding sites for GTP, one of which overlaps with the tail end of the N-t...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"