重组
RabMAb

Anti-Rho抗体[Y486] (ab32046)

概述

  • 产品名称
    Anti-Rho抗体[Y486]
    参阅全部 Rho 一抗
  • 描述
    兔单克隆抗体[Y486] to Rho
  • 特异性
    The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the N-term of human Rho.

  • 阳性对照
    • PC12 cell lysate and hepatocellular carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-Rho antibody (Alexa Fluor® 488) [Y486] (ab203324)

    Anti-Rho antibody (Alexa Fluor® 647) [Y486] (ab203325)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab32046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    • 序列相似性
      Belongs to the small GTPase superfamily. Rho family.
    • 结构域
      The basic-rich region is essential for yopT recognition and cleavage.
    • 翻译后修饰
      Substrate for botulinum ADP-ribosyltransferase.
      Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
      AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
      Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
      Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
    • 细胞定位
      Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Cytoplasm > cell cortex. Midbody. Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARH12 antibody
      • ARHA antibody
      • H12 antibody
      • ras homolog gene family member A antibody
      • ras homolog gene family member B antibody
      • ras homolog gene family member C antibody
      • Rho cDNA clone 12 antibody
      • RHO12 antibody
      • RHOA antibody
      • RHOA_HUMAN antibody
      • rhob antibody
      • rhoc antibody
      • RHOH12 antibody
      • Small GTP binding protein RhoA antibody
      • Transforming protein RhoA antibody
      see all

    图片

    • Anti-Rho antibody [Y486] (ab32046) at 1/2000 dilution + PC12 cell lysate

      Predicted band size : 21 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab32046 at 1/100 dilution.

    文献

    This product has been referenced in:
    • Greenaway JB  et al. Ovarian tumour growth is characterized by mevalonate pathway gene signature in an orthotopic, syngeneic model of epithelial ovarian cancer. Oncotarget 7:47343-47365 (2016). WB, IF . Read more (PubMed: 27329838) »
    • Girouard MP  et al. RhoA Proteolysis Regulates the Actin Cytoskeleton in Response to Oxidative Stress. PLoS One 11:e0168641 (2016). Read more (PubMed: 27992599) »

    See all 4 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (lung epithelial cells)
    Specification
    lung epithelial cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 31 2012

    Thank you for your enquiry. I have been in touch with the lab for their comments. Unfortunately this has not been tested. From a literature search I can see that there are 3 binding sites for GTP, one of which overlaps with the tail end of the N-t...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册