概述

  • 产品名称
  • 描述
    兔多克隆抗体to Rho
  • 特异性
    This antibody recognizes ~24 kDa of human Rho A protein. It also reacts with mouse and rat.
  • 经测试应用
    适用于: IHC-P, WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A synthetic peptide corresponding to the C-terminus of human Rho A protein (P61586).

  • 阳性对照
    • MCF7 Cell Lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Proclin
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

靶标

  • 功能
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rho family.
  • 结构域
    The basic-rich region is essential for yopT recognition and cleavage.
  • 翻译后修饰
    Substrate for botulinum ADP-ribosyltransferase.
    Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
    AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
    Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
    Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • 细胞定位
    Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Cytoplasm > cell cortex. Midbody. Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARH12 antibody
    • ARHA antibody
    • H12 antibody
    • ras homolog gene family member A antibody
    • ras homolog gene family member B antibody
    • ras homolog gene family member C antibody
    • Rho cDNA clone 12 antibody
    • RHO12 antibody
    • RHOA antibody
    • RHOA_HUMAN antibody
    • rhob antibody
    • rhoc antibody
    • RHOH12 antibody
    • Small GTP binding protein RhoA antibody
    • Transforming protein RhoA antibody
    see all

Anti-Rho antibody 图像

  • All lanes : Anti-Rho antibody (ab66124) at 1/500 dilution

    Lane 1 : MCF7 Cell Lysate with immunising peptide
    Lane 2 : MCF7 Cell Lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

Anti-Rho antibody (ab66124)参考文献

ab66124 has not yet been referenced specifically in any publications.

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