概述

  • 产品名称Anti-RHEB抗体[EPR2971]
    参阅全部 RHEB 一抗
  • 描述
    兔单克隆抗体[EPR2971] to RHEB
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human RHEB

  • 阳性对照
    • Raji, SH-SY5Y cell lysates and Human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-RHEB antibody (Biotin) [EPR2971] (ab199747)

    Anti-RHEB antibody (HRP) [EPR2971] (ab199230)

性��

应用

Our Abpromise guarantee covers the use of ab92313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Predicted molecular weight: 20 kDa.Can be blocked with Human RHEB peptide (ab194764).
IHC-P 1/100 - 1/250.

Heat mediated antigen retrieval and use of an HRP/AP polymerized secondary antibody recommended.

ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
    • 组织特异性Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
    • 序列相似性Belongs to the small GTPase superfamily. Rheb family.
    • 翻译后修饰Farnesylation is important for efficiently activating mTORC1-mediated signaling.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ras homolog enriched in brain 2, formerly antibody
      • GTP binding protein Rheb antibody
      • GTP-binding protein Rheb antibody
      • MGC111559 antibody
      • Ras homolog enriched in brain 2 antibody
      • Ras homolog enriched in brain antibody
      • RHEB 2 antibody
      • Rheb antibody
      • RHEB_HUMAN antibody
      • RHEB2 antibody
      • RHEB2, formerly antibody
      see all

    Anti-RHEB antibody [EPR2971] 图像

    • Overlay histogram showing Raji cells stained with ab92313 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92313, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-RHEB antibody [EPR2971] (ab92313) at 1/2000 dilution

      Lane 1 : Raji cell lsyate
      Lane 2 : SH-SY5Y cell lsyate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 20 kDa
    • ab92313, at 1/100 dilution, staining RHEB in paraffin-embedded Human brain tissue, by Immunohistochemistry.

    Anti-RHEB antibody [EPR2971] (ab92313)参考文献

    This product has been referenced in:
    • Jiang L  et al. Rheb/mTORC1 Signaling Promotes Kidney Fibroblast Activation and Fibrosis. J Am Soc Nephrol 24:1114-26 (2013). IHC-P ; Human . Read more (PubMed: 23661807) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab92313.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"