概述

  • 产品名称Anti-RHEB抗体
    参阅全部 RHEB 一抗
  • 描述
    兔多克隆抗体to RHEB
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RHEB.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Raji; SHSY5Y; EGF treated A431; Jurkat.

性能

应用

Our Abpromise guarantee covers the use of ab109914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).

靶标

  • 功能Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
  • 组织特异性Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
  • 序列相似性Belongs to the small GTPase superfamily. Rheb family.
  • 翻译后修饰Farnesylation is important for efficiently activating mTORC1-mediated signaling.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ras homolog enriched in brain 2, formerly antibody
    • GTP binding protein Rheb antibody
    • GTP-binding protein Rheb antibody
    • MGC111559 antibody
    • Ras homolog enriched in brain 2 antibody
    • Ras homolog enriched in brain antibody
    • RHEB 2 antibody
    • Rheb antibody
    • RHEB_HUMAN antibody
    • RHEB2 antibody
    • RHEB2, formerly antibody
    see all

Anti-RHEB antibody 图像

  • All lanes : Anti-RHEB antibody (ab109914) at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : EGF-Stimulated A431 Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,49 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-RHEB antibody (ab109914)参考文献

ab109914 has not yet been referenced specifically in any publications.

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