The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Use at an assay dependent concentration. PubMed: 24648344
相关性RHBDF1 is a seven-transmembrane protein with a long N-terminal cytoplasmic extension that comprises half of the polypeptide sequence, and is found in the endoplasmic reticulum and Golgi, but not on the cell surface. RHBDF1 has a pivotal role in sustaining growth signals in epithelial cancer cells and thus may serve as a therapeutic target for treating epithelial cancers.
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Anti-RHBDF1 antibody 图像
Western blot - RHBDF1 antibody (ab81342)
Anti-RHBDF1 antibody (ab81342) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 97 kDa
Anti-RHBDF1 antibody (ab81342)参考文献
This product has been referenced in:
Zhou Z et al. Human rhomboid family-1 suppresses oxygen-independent degradation of hypoxia-inducible factor-1a in breast cancer. Cancer Res74:2719-30 (2014).
Read more (PubMed: 24648344) »