重组Anti-RGS9抗体[EPR2873] (ab108975)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2873] to RGS9
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RGS9抗体[EPR2873]
参阅全部 RGS9 一抗 -
描述
兔单克隆抗体[EPR2873] to RGS9 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Y79 and PC12 cell lysates and human and mouse spleen tissue lysates. ICC/IF: Neuro-2a cells. Flow Cyt (intra): PC12 and Y79 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2873 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108975于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/30 - 1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa).
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ICC/IF |
1/50 - 1/100.
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说明 |
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Flow Cyt (Intra)
1/30 - 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 77 kDa). |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction. -
组织特异性
Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors). -
疾病相关
Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS) [MIM:608415]; also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones. -
序列相似性
Contains 1 DEP domain.
Contains 1 G protein gamma domain.
Contains 1 RGS domain. -
结构域
In photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments. -
翻译后修饰
Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition. -
细胞定位
Membrane. Isoform 3 is targeted to the membrane via its interaction with RGS9BP. - Information by UniProt
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数据库链接
- Entrez Gene: 8787 Human
- Entrez Gene: 19739 Mouse
- Entrez Gene: 29481 Rat
- Omim: 604067 Human
- SwissProt: O75916 Human
- SwissProt: O54828 Mouse
- SwissProt: P49805 Rat
- Unigene: 664380 Human
see all -
别名
- PERRS antibody
- R9AP antibody
- Regulator of G protein signalling 9 antibody
see all
图片
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All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified)
Lane 1 : Y79 whole cell lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (purified) + PC-12 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Intracellular Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluorr® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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All lanes : Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution (unpurified)
Lane 1 : PC12 lysates
Lane 2 : Human spleen lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Note: Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4. -
Intracellular flow cytometric analysis ofpermeabilized PC12 cells labelling RGS9with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108975 被引用在 1 文献中.
- Vogt A et al. Isotope coded protein labeling coupled immunoprecipitation (ICPL-IP): a novel approach for quantitative protein complex analysis from native tissue. Mol Cell Proteomics 12:1395-406 (2013). PubMed: 23268931