Anti-RGS4抗体(ab97307)
Key features and details
- Rabbit polyclonal to RGS4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-RGS4抗体
参阅全部 RGS4 一抗 -
描述
兔多克隆抗体to RGS4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-198 of Human RGS4 (AAH00737)
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阳性对照
- WB: Raji cell lysate, rat and mouse brain tissue extract; IHC-P: Human breast cancer and mouse muscle tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97307于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
靶标
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功能
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. -
疾病相关
Genetic variation in RGS4 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. -
序列相似性
Contains 1 RGS domain. -
翻译后修饰
Palmitoylated on Cys-2 and/or Cys-12.
Phosphorylated by cyclic GMP-dependent protein kinase. - Information by UniProt
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数据库链接
- Entrez Gene: 5999 Human
- Entrez Gene: 19736 Mouse
- Entrez Gene: 29480 Rat
- Omim: 602516 Human
- SwissProt: P49798 Human
- SwissProt: O08899 Mouse
- SwissProt: P49799 Rat
- Unigene: 386726 Human
see all -
别名
- MGC2124 antibody
- MGC60244 antibody
- Regulator of G protein signalling 4 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining RGS4 with ab97307 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa
12% SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded mouse muscle tissue staining RGS4 with ab97307 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Mouse cerebellum tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
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Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97307 被引用在 1 文献中.
- Xiao D & Gao HX Mechanism of miR-107-targeting of regulator of G-protein signaling 4 in hepatocellular carcinoma. Oncol Lett 18:5145-5154 (2019). PubMed: 31612026