概述

  • 产品名称Anti-RGS2抗体
    参阅全部 RGS2 一抗
  • 描述
    兔多克隆抗体to RGS2
  • 特异性PPlease note that a patterns of "background" bands may be present, this varies from tissue to tissue.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    KREKMKRTLLKDWKTRC

    , corresponding to N terminal amino acids 29-44 of Human RGS2.

性能

应用

Our Abpromise guarantee covers the use of ab9963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/15000. Predicted molecular weight: 25 kDa.

靶标

  • 功能Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
  • 组织特异性Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
  • 序列相似性Contains 1 RGS domain.
  • 翻译后修饰Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.
  • 细胞定位Cell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic helix-loop-helix phosphoprotein G0S8 antibody
    • Cell growth inhibiting protein 31 antibody
    • Cell growth-inhibiting gene 31 protein antibody
    • G0 to G1 switch regulatory 8 24kD antibody
    • G0/G1 switch regulatory protein 8 antibody
    • G0S8 antibody
    • GOS8 antibody
    • OTTHUMP00000060765 antibody
    • Regulator of G protein signaling 2 antibody
    • Regulator of G protein signalling 2 24kD antibody
    • Regulator of G-protein signaling 2 antibody
    • Regulators of G protein signaling XRGSVI antibody
    • RGS 2 antibody
    • RGS2 antibody
    • RGS2_HUMAN antibody
    see all

Anti-RGS2 antibody (ab9963)参考文献

This product has been referenced in:
  • Snabaitis AK  et al. Regulation of the extracellular signal-regulated kinase pathway in adult myocardium: differential roles of G(q/11), Gi and G(12/13) proteins in signalling by alpha1-adrenergic, endothelin-1 and thrombin-sensitive protease-activated receptors. Cell Signal 17:655-64 (2005). WB ; Rat . Read more (PubMed: 15683740) »
  • Kveberg L  et al. Expression of regulator of G protein signalling proteins in natural killer cells, and their modulation by Ly49A and Ly49D. Immunology 115:358-65 (2005). Read more (PubMed: 15946253) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. I am sorry to hear that the antibody is giving you problems. As we discussed over the phone, potential troubleshooting tips that could help improve your results are: 1) loading more protein per lane (up to 100 ug max...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse ventricle)
Loading amount 50 µg
Specification mouse ventricle
Gel Running Conditions Reduced Denaturing (12% gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Serban Georgescu

Verified customer

提交于 Apr 21 2008

I received confirmation today that the patterns of "background" bands varies from tissue to tissue. The VTA area of the brain has not been tested in the tester's hands therefore we do not have experience of a 12kDa band, but in other tissues other band...

Read More

Thank you for taking the time to submit these concerns to me. If there are only two bands on the blot and background this problem may be due in part to target breakdown or standard cross reactivity due to the fact that the product is a pAb. Since pAbs ...

Read More

Thank you for your patience. The originator of the antibody has never looked at 3T3 cells (which are fibroblasts), but at the RNA level RGS2 is most highly expressed in brain. In 3T3 cells, RGS2 mRNA may be low, or the protein may be unstable. The l...

Read More

Thank you for your phone call. I'm sending you a free of charge replacement for ab9963, for your record it is on order# 50879 and you should receive it Tuesday. The vial is from lot 27062 but I checked it and it is fine. Please let me know if you have ...

Read More

Thank you for your enquiry. Ab9963 has been tested only against transfected or purified recombinant RGS2. If you have any more questions, please contact us again.

Thank you for your patience. Unfortunately I still have not heard from the originator of this antibody regarding your questions about the multiple bands. As for the background that you are experiencing, high background is usually a sign for blocking pr...

Read More

Thank you for your enquiry and your interest in our products. All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). Our policy at Abcam is that if an ant...

Read More

Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"