The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 24 kDa.
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).
组织特异性Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).
序列相似性Contains 1 RGS domain.
翻译后修饰Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.
细胞定位Cell membrane. Mitochondrion and Cell membrane. Cytoplasm. Nucleus, nucleolus.
ab115684 at 20µg/ml staining RGS2 in Formalin-fixed, Paraffin-embedded Human brain (cortex) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-RGS2 antibody (ab115684)参考文献
has not yet been referenced specifically in any publications.