Anti-RGS19抗体(ab72085)

概述

  • 产品名称Anti-RGS19抗体
    参阅全部 RGS19 一抗
  • 描述
    兔多克隆抗体to RGS19
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A portion of amino acids 1-50 of human RGS19 (NP_005864).

  • 阳性对照
    • Ramos, mouse placenta lysate and human liver tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects bands of approximately 25 and 50 kDa (predicted molecular weight: 25 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.
    • 组织特异性Highest expression in lung. Placenta, liver and heart also express high levels of GAIP.
    • 序列相似性Contains 1 RGS domain.
    • 翻译后修饰Fatty acylated. Heavily palmitoylated in the cysteine string motif.
      Phosphorylated, mainly on serine residues.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • G alpha interacting protein antibody
      • G protein signalling regulator 19 antibody
      • G protein, alpha-interacting protein antibody
      • G-alpha-interacting protein antibody
      • GAIP antibody
      • GNAI3IP antibody
      • Guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein antibody
      • Regulator of G protein signalling 19 antibody
      • Regulator of G-protein signaling 19 antibody
      • RGS19 antibody
      • RGS19_HUMAN antibody
      • RGSGAIP antibody
      see all

    Anti-RGS19 antibody 图像

    • All lanes : Anti-RGS19 antibody (ab72085) at 1 µg/ml

      Lane 1 : Mouse placenta lysate
      Lane 2 : Mouse placenta lysate plus immunizing peptide


      Predicted band size : 25 kDa
      Observed band size : 25 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of RGS19 in paraffin embedded, formalin fixed human liver tissue using an isotype control (top) and ab72085 (bottom) at 5µg/ml.

    Anti-RGS19 antibody (ab72085)参考文献

    ab72085 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (A549, HeLa, and HEK293)
    Specification A549, HeLa, and HEK293
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Mr. Junmo Hwang

    Verified customer

    提交于 Mar 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"