Anti-RGS14抗体(ab96674)
Key features and details
- Rabbit polyclonal to RGS14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RGS14抗体
参阅全部 RGS14 一抗 -
描述
兔多克隆抗体to RGS14 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 504-566 of Human RGS14 (NP_006471).
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阳性对照
- MOLT4 and Raji cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
靶标
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功能
Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. -
序列相似性
Contains 1 GoLoco domain.
Contains 2 RBD (Ras-binding) domains.
Contains 1 RGS domain. -
结构域
The RGS domain is necessary for GTPase-activating protein (GAP) activity for G subunits and localization to the nucleus and centrosomes.
The GoLoco domain is necessary for GDP-dissociation inhibitor (GDI) activity, translocation out of the nucleus and interaction with G(i) alpha subunits GNAI1, GNAI2 and GNAI3.
The RBD domains are necessary for localization to the nucleus and centrosomes. -
翻译后修饰
Phosphorylated by PKC. Phosphorylation is increased in presence of forskolin and may enhance the GDI activity on G(i) alpha subunit GNAI1. -
细胞定位
Nucleus. Nucleus > PML body. Cytoplasm. Membrane. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel-shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compartment near the microtubule-organizing center (MTOC). Expressed in the nucleus during interphase and segregates to the centrosomes and astral MTs during mitosis. Relocalizes to the nucleus in PML nuclear bodies in response to heat stress. Colocalizes with RIC8A in CA2 hippocampal neurons. Localizes to spindle poles during metaphase. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. Recruited from the cytosol to the plasma membrane by the inactive GDP-bound forms of G(i) alpha subunits GNAI1 and GNAI3. Recruited from the cytosol to membranes by the active GTP-bound form of HRAS1. Colocalizes with G(i) alpha subunit GNAI1 and RIC8A at the plasma membrane. Colocalizes with BRAF and RAF1 in both the cytoplasm and membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 10636 Human
- Omim: 602513 Human
- SwissProt: O43566 Human
- Unigene: 9347 Human
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别名
- Highly similar to rap1/rap2 interacting protein antibody
- OTTHUMP00000223586 antibody
- OTTHUMP00000223587 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab96674 被引用在 4 文献中.
- Friedman PA et al. RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein. J Biol Chem 298:101836 (2022). PubMed: 35307350
- Sevim Ç et al. An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas. Toxicol Rep 6:42-50 (2019). PubMed: 30560060
- Çomakli S & Özdemir S Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1. Pathogens 8:N/A (2019). PubMed: 30823555
- Özdemir S et al. Imidacloprid exposure cause the histopathological changes, activation of TNF-a, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpioL.). Toxicol Rep 5:125-133 (2018). PubMed: 29321977