Anti-RGNEF抗体(ab122399)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to RGNEF
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen: GTAHTEAQQS FMSPSSSCAS NLNLSFGWHG FEKEQSHLKK RSSSLDALDA DSEGEGHSEP SHICYTPGSQ SSSRTGIPSG DELDSFETNT EPDFNI, corresponding to amino acids 435-530 of Human RGNEF according to Q8N1W1

  • 阳性对照
    • Human duodenum tissue, Human U-2 OS cell line.

性能

应用

Our Abpromise guarantee covers the use of ab122399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

  • 功能
    Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.
  • 序列相似性
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • 翻译后修饰
    Phosphorylated on tyrosine upon stimulation of cells by laminin.
  • 细胞定位
    Cytoplasm. Cell membrane. Colocalizes with the microtubule radial and cortical systems.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 190 kDa guanine nucleotide exchange factor antibody
    • guanine nucleotide exchange factor, 190-KD antibody
    • KIAA1998 antibody
    • p190-RhoGEF antibody
    • p190RhoGEF antibody
    • RGNEF antibody
    • RGNEF, mouse, homolog of antibody
    • RGNEF_HUMAN antibody
    • Rho guanine nucleotide exchange factor (GEF) 28 antibody
    • rho guanine nucleotide exchange factor 28 antibody
    • Rho interacting protein 2 antibody
    • Rho-guanine nucleotide exchange factor antibody
    • RhoGEF antibody
    • RIP2 antibody
    see all

Anti-RGNEF antibody 图像

  • ab122399, at a 1/200 dilution, staining RGNEF in glandular cells of paraffin-embedded Human duodenum tissue by immunohistochemistry.
  • ab122399, at 4 µg/ml, staining RGNEF (in green) in PFA/Triton X-100 treated Human cell line U-2 OS by immunofluorescence.

Anti-RGNEF antibody (ab122399)参考文献

ab122399 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (3T3 Fibroblasts)
Permeabilization
Yes - 0.1% Triton-X
Specification
3T3 Fibroblasts
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册