Anti-RFX5抗体(ab9255)
Key features and details
- Rabbit polyclonal to RFX5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RFX5抗体
参阅全部 RFX5 一抗 -
描述
兔多克隆抗体to RFX5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide (Human) conjugated to KLH - which represents aportion of human Regulatory Factor X 5 (GenBank PID 4557543).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
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说明 |
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WB
1/500 - 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). |
靶标
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功能
Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. -
组织特异性
Ubiquitous. -
疾病相关
Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. -
序列相似性
Belongs to the RFX family.
Contains 1 RFX-type winged-helix DNA-binding domain. -
结构域
The N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y. -
翻译后修饰
Phosphorylated. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5993 Human
- Omim: 601863 Human
- SwissProt: P48382 Human
- Unigene: 632472 Human
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别名
- DNA binding protein RFX 5 antibody
- DNA binding protein RFX5 antibody
- DNA-binding protein RFX5 antibody
see all
图片
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ab9255 at a 1:1000 dilution staining RFX5 in 500
µ g of HeLa Nuclear Extract by Western Blot (ECL). Immunoprecipitation was performed prior to Western Blotting with 2.0µ g of ab9255.
ab9255 at a 1:1000 dilution staining RFX5 in 500µg of HeLa Nuclear Extract by Western Blot (ECL). Immunoprecipitation was performed prior to Western Blotting with 2.0µg of ab9255.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab9255 被引用在 4 文献中.
- Shen H et al. ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression. Cell Death Dis 12:349 (2021). PubMed: 33824282
- Mueller PA et al. Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages. Circulation 138:1850-1863 (2018). PubMed: 29794082
- Chen L et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). PubMed: 29212196
- Wolf K et al. Treatment of Chlamydia trachomatis with a small molecule inhibitor of the Yersinia type III secretion system disrupts progression of the chlamydial developmental cycle. Mol Microbiol 61:1543-55 (2006). PubMed: 16968227