概述

  • 产品名称Anti-RFX5抗体
    参阅全部 RFX5 一抗
  • 描述
    兔多克隆抗体to RFX5
  • 特异性Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa.
  • 经测试应用适用于: ELISA, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 320-494 of Human RFX 5.

  • 常规说明Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab7542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).
EMSA Use a concentration of 0.5 - 1 µg/ml.

靶标

  • 功能Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • 序列相似性Belongs to the RFX family.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • 结构域The N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA binding protein RFX 5 antibody
    • DNA binding protein RFX5 antibody
    • DNA-binding protein RFX5 antibody
    • Influences HLA class II expression antibody
    • Regulatory factor X 5 antibody
    • Regulatory factor X subunit 5 antibody
    • Regulatory factor X, 5 (influences HLA class II expression) antibody
    • RFX 5 antibody
    • RFX5 antibody
    • Rfx5 protein antibody
    • RFX5_HUMAN antibody
    see all

Anti-RFX5 antibody (ab7542)参考文献

ab7542 has not yet been referenced specifically in any publications.

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