概述

  • 产品名称Anti-RFX5抗体
    参阅全部 RFX5 一抗
  • 描述
    兔多克隆抗体to RFX5
  • 特异性Human RFX5 from a variety of fibroblast and B-cell lysates. Recognition is at the C-terminal region.
  • 经测试应用适用于: WB, IP, ELISA, EMSAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human).

  • 常规说明Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by delipidation and defibrination.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab6557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
EMSA
  • 应用说明EMSA: Use 0.5 to 1.0 ml per assay.
    ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: 1/250. Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
    • 组织特异性Ubiquitous.
    • 疾病相关Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
    • 序列相似性Belongs to the RFX family.
      Contains 1 RFX-type winged-helix DNA-binding domain.
    • 结构域The N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
    • 翻译后修饰Phosphorylated.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA binding protein RFX 5 antibody
      • DNA binding protein RFX5 antibody
      • DNA-binding protein RFX5 antibody
      • Influences HLA class II expression antibody
      • Regulatory factor X 5 antibody
      • Regulatory factor X subunit 5 antibody
      • Regulatory factor X, 5 (influences HLA class II expression) antibody
      • RFX 5 antibody
      • RFX5 antibody
      • Rfx5 protein antibody
      • RFX5_HUMAN antibody
      see all

    Anti-RFX5 antibody (ab6557)参考文献

    ab6557 has not yet been referenced specifically in any publications.

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