概述

  • 产品名称Anti-RFX3抗体
    参阅全部 RFX3 一抗
  • 描述
    兔多克隆抗体to RFX3
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to an internal sequence of Human RFX3.

  • 阳性对照
    • IHC-P: Human colon carcinoma tissue; WB: extracts from RAW264.7 or COLO cells.

性能

应用

Our Abpromise guarantee covers the use of ab92670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/40000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 84 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.
    • 序列相似性Belongs to the RFX family.
      Contains 1 H-T-H motif winged-type DNA-binding domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bA32F11.1 antibody
      • DNA binding protein RFX3 antibody
      • Regulatory factor X 3 antibody
      • Regulatory factor X, 3 (influences HLA class II expression) antibody
      • RFX3 antibody
      • RFX3_HUMAN antibody
      • Transcription factor RFX3 antibody
      see all

    Anti-RFX3 antibody 图像

    • All lanes : Anti-RFX3 antibody (ab92670) at 1/500 dilution

      Lane 1 : extracts from RAW264.7 cells
      Lane 2 : extracts from COLO cells
      Lane 3 : extracts from RAW264.7 cells with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
    • Left panel: ab92670 at 1/50 dilution staining RFX3 in paraffin-embedded Human colon carcinoma tissue.
      Right panel: same, after pre-incubation with the immunizing peptide.

    Anti-RFX3 antibody (ab92670)参考文献

    ab92670 has not yet been referenced specifically in any publications.

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