Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human RFX2.
(Peptide available as ab89390.)
- This antibody gave a positive signal in the HeLa nuclear whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 80 kDa).
Belongs to the RFX family.
Contains 1 RFX-type winged-helix DNA-binding domain.
Information by UniProt
- DNA-binding protein RFX2 antibody
- HLA class II regulatory factor RFX2 antibody
- regulatory factor X 2 (influences HLA class II expression) antibody
Western blot - RFX2 antibody (ab79241)
Anti-RFX2 antibody (ab79241) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µgSecondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size :
80 kDaObserved band size :
82 kDa (why is the actual band size different from the predicted?
)Additional bands at :
98 kDa. We are unsure as to the identity of these extra bands.Exposure time :
has not yet been referenced specifically in any publications.
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