概述

  • 产品名称Anti-RFWD3抗体
    参阅全部 RFWD3 一抗
  • 描述
    兔多克隆抗体to RFWD3
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Immunogen corresponds to a region within amino acids 75-125 of Human RFWD3 (NP_060594.3).

  • 阳性对照
    • Hela whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG

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应用

Our Abpromise guarantee covers the use of ab99306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Contains 1 RING-type zinc finger.
      Contains 3 WD repeats.
    • 翻译后修饰Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BC027246 antibody
      • E3 ubiquitin protein ligase RFWD3 antibody
      • E3 ubiquitin-protein ligase RFWD3 antibody
      • FLJ10520 antibody
      • MGC27888 antibody
      • RFWD3 antibody
      • RFWD3_HUMAN antibody
      • Ring finger and WD repeat domain 3 antibody
      • RING finger and WD repeat domain containing protein 3 antibody
      • RING finger and WD repeat domain-containing protein 3 antibody
      • RING finger protein 201 antibody
      • RNF201 antibody
      see all

    Anti-RFWD3 antibody 图像

    • An anti RFWD3 antibody, at 1 µg/ml, staining RFWD3 in HeLa whole cell lysate immunoprecipitated using ab99306 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-RFWD3 antibody (ab99306)参考文献

    ab99306 has not yet been referenced specifically in any publications.

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