Synthetic peptide corresponding to a region within the internal sequence amino acids 72-121 (MTKQHNSRLE QSLHVREELR HFREDVTLDA ATASSLLVFS NDLRSAQCKK) of human RFPL4B (NP_001013756).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:62500.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
Information by UniProt
- Ret finger protein like 4B antibody
- Ret finger protein-like 4B antibody
- RFPL4B antibody
Western blot - RFPL4B antibody (ab83526)
Anti-RFPL4B antibody (ab83526) at 1 µg/ml (in 5% skim milk/ PBS) + HeLa cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
30 kDaObserved band size :
35 kDa (why is the actual band size different from the predicted?
)Additional bands at :
85 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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