概述

  • 产品名称
    Anti-RFPL4B抗体
  • 描述
    兔多克隆抗体to RFPL4B
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 72-121 (MTKQHNSRLE QSLHVREELR HFREDVTLDA ATASSLLVFS NDLRSAQCKK) of human RFPL4B (NP_001013756).

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

靶标

图片

  • Anti-RFPL4B antibody (ab83526) at 1 µg/ml (in 5% skim milk/ PBS) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab83526 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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