WB, ELISAmore details
Synthetic peptide corresponding to a region within the internal sequence amino acids 72-121 (MTKQHNSRLE QSLHVREELR HFREDVTLDA ATASSLLVFS NDLRSAQCKK) of human RFPL4B (NP_001013756).
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
- 纯化说明Purified by peptide affinity chromatography method.
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:62500.
- 序列相似性Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
Information by UniProt
- Ret finger protein like 4B antibody
- Ret finger protein-like 4B antibody
- RFPL4B antibody
Anti-RFPL4B antibody 图像
Western blot - RFPL4B antibody (ab83526)
Anti-RFPL4B antibody (ab83526) at 1 µg/ml (in 5% skim milk/ PBS) + HeLa cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 30 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
Anti-RFPL4B antibody (ab83526)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"