概述

  • 产品名称Anti-RFP2抗体
    参阅全部 RFP2 一抗
  • 描述
    兔多克隆抗体to RFP2
  • 特异性This antibody is specific for RFP2.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    A region within synthetic peptide: DTLETSKRKS LQLLTKDSDK VKEFFEKLQH TLDQKKNEIL SDFETMKLAV, corresponding to amino acids 162-211 of Human RFP2

  • 阳性对照
    • Fetal lung lysate, human kidney tissue and human lung tissue.

性能

应用

Our Abpromise guarantee covers the use of ab42502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 39, 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 RING-type zinc finger.
  • 结构域The coiled-coil domain is required for the induction of autophagy during endoplasmic reticulum (ER) stress.
    The RING-type zinc finger is required for auto-polyubiquitination.
    The C-terminal domain transmembrane domain is indispensable for the localization to the ER.
  • 翻译后修饰Auto-ubiquitinated; requires the RING-type zinc finger. Auto-polyubiquitination leads to proteasomal degradation.
  • 细胞定位Endoplasmic reticulum membrane. Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell chronic lymphocytic leukemia tumor suppressor Leu5 antibody
    • B-cell chronic lymphocytic leukemia tumor suppressor Leu5 antibody
    • CAR antibody
    • DLEU5 antibody
    • E3 ubiquitin-protein ligase TRIM13 antibody
    • HGNC:9976 antibody
    • LEU5 antibody
    • Leukemia associated protein 5 antibody
    • Leukemia-associated protein 5 antibody
    • Putative tumor suppressor RFP2 antibody
    • Ret finger protein 2 antibody
    • RING finger protein 77 antibody
    • RNF77 antibody
    • TRI13_HUMAN antibody
    • Trim13 antibody
    • Tripartite motif protein 13 antibody
    • Tripartite motif-containing protein 13 antibody
    see all

Anti-RFP2 antibody 图像

  • Anti-RFP2 antibody (ab42502) at 0.125 µg/ml + Fetal lung lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 39& 47 kDa (why is the actual band size different from the predicted?)
  • ab42502 at 4.0µg/ml staining RFP2 in human alveolar cells, lung tissue.
  • ab42502 at 4.0µg/ml staining RFP2 in human epithelial cells of collecting tubule in kidney tissue.

Anti-RFP2 antibody (ab42502)参考文献

ab42502 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab42502.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"