Anti-RFP抗体[RF5R] (ab125244)
Key features and details
- Mouse monoclonal [RF5R] to RFP
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-RFP抗体[RF5R]
参阅全部 RFP 一抗 -
描述
小鼠单克隆抗体[RF5R] to RFP -
宿主
Mouse -
特异性
Recognizes native and denatured forms of RFP and its turbo-RFP, DsRed variants
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Transfected HEK293 cell lysate.
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常规说明
Store at 4°C (add 0.1% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab125244 is purified by protein A affinity chromatography from TCS -
克隆
单克隆 -
克隆编号
RF5R -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125244于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/3000. Predicted molecular weight: 26 kDa.
Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins. |
说明 |
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WB
1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins. |
靶标
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相关性
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
别名
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
图片
实验方案
数据表及文件
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Datasheet download
文献 (11)
ab125244 被引用在 11 文献中.
- Su M et al. Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors. Mol Brain 14:67 (2021). PubMed: 33845857
- Pereira Luppi M et al. Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins. Cell Rep 37:109975 (2021). PubMed: 34758317
- Leung C et al. Lgr5 Marks Adult Progenitor Cells Contributing to Skeletal Muscle Regeneration and Sarcoma Formation. Cell Rep 33:108535 (2020). PubMed: 33357435
- Kueck T et al. Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. J Virol 93:N/A (2019). PubMed: 31578292
- Seishima R et al. Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development. Nat Commun 10:5378 (2019). PubMed: 31772170