概述

  • 产品名称Anti-RFP抗体(HRP)
    参阅全部 RFP 一抗
  • 描述
    兔多克隆抗体to RFP (HRP)
  • 偶联物HRP
  • 特异性ab34767 is designed to detect RFP and its variants.
  • 经测试应用适用于: ELISA, WBmore details
  • 免疫原

    Fusion protein The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明immunoaffinity chromatography was followed by solid phase adsorption to remove any unwanted reactivities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000. Detects a band of approximately 27 kDa.

靶标

  • 相关性Red fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
  • 别名
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • Red fluorescent protein eqFP611 antibody
    • RFP antibody

Anti-RFP antibody (HRP) 图像

  • All lanes : Anti-RFP antibody (HRP) (ab34767) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

Anti-RFP antibody (HRP) (ab34767)参考文献

This product has been referenced in:
  • Thevenard J  et al. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities. PLoS One 9:e103839 (2014). WB . Read more (PubMed: 25075518) »
  • Chen XW  et al. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife 2:e00444 (2013). Read more (PubMed: 23580231) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Escherichia coli Cell lysate - whole cell (E. coli expressing mCherry)
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Loading amount 30 cells
Specification E. coli expressing mCherry
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 20 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (Prostate)
Specification Prostate
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 24 2013

Thank you very much for your interest in ab34767.

To our knowledge, ab34767 has not been tested in tdTomato, however based on sequence homology there is a good chance that it will work. Therefore, I can offer a discount off a future purchase...

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Thank you for your reply. I have sent a free vial of ab34767 to replace ab28664. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. To check the status of the order please contact our Cu...

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Thank you for your email. I would be happy to send a free vial of ab34767 (to replace ab28664) aswell as credit you for the secondary antibody purchased. Do you have the secondary product code and date of order? I need to find the original order...

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Thank you for the further information. My colleague Kate is currently out of the office, so I will be managing this case. I am sorry to hear you have been experincing problems with ab28664. I would be happy to send you a replacment of another pr...

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Thank you for your enquiry. Yes, ab28664 has been tested for Western blotting and will demonstrate the expression of your DSred fusion protein. But I recommend ab34767 which is an HRP conjugate, removing the need for a secondary detection antibody....

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Thank you for your recent technical enquiry. Unfortunately, we do not have information regarding the reactivity of this product with mcherry. It has only been shown to detect RFP from Discosoma. I am sorry I can't be of more assistance in this o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"