概述

  • 产品名称
    Anti-RFP抗体(FITC)
    参阅全部 RFP 一抗
  • 描述
    兔多克隆抗体to RFP (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    ab34764 detects RFP tagged proteins. No reaction was observed against serum proteins.Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry”.
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, WB, ELISAmore details
  • 免疫原

    Fusion protein corresponding to RFP. derived from the mushroom polyp coral Discosoma

  • 常规说明

    3.4 moles FITC per mole of rabbit IgG.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    affinity purification was followed by solid phase adsorption to remove any unwanted reactivities.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/500 - 1/2500.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/2500.
WB 1/10000. Detects a band of approximately 27 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • 别名
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

图片

  • All lanes : Anti-RFP antibody (FITC) (ab34764) at 1/2500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

文献

This product has been referenced in:
  • Donnelly JM  et al. Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach. PLoS One 8:e75225 (2013). Read more (PubMed: 24069395) »
  • Starremans PG  et al. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73:1394-405 (2008). Read more (PubMed: 18385665) »

See all 3 Publications for this product

客户评价及客户问答

We have not yet tested the FITC conjugated anti RFP antibody against GFP, but this antibody did not show significant amounts of specific binding to BFP.

DISCOUNT CODE: ABTRIAL-xxxxx
Expiration date: 04/04/12
Value: €410.00

I am very pleased to hear you would like to accept our offer and test ab34771 in Flow Cytometry. This code will give you €410.00 off your next order before...

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Thank you for your answer.

I would be pleased to offer you the AbTrial for the ab34771 to use this antibody in FlowCytometry. If you decide to order this antibody please let me now in advance so that I can provide you with the code.

Y...

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Thank you for contacting us.

In this case I would recommend to use the Anti-RFP antibody (ab62341). If it is suitable for your experiment you could use a secondary antibody to detect the Anti RFP antibody.

If the conjugate needs to be...

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Thank you for your enquiry.

This Our RFP antibody isdesigned to detect all RFP variants from Discosoma. I am sorry we do not have specific data for the the use of this product with DS Red express in Flow Cytometry, however in theory it would...

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Thank you for contacting us with your question.

We have not specifically tested the antibody against mRFP1, but it is designed to react with RFP variants such as mRFP1. If you do have any problems using this antibody with mRPF1, please let me...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (whole embryo)
Specification
whole embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - PBT
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Vanja Stankic

Verified customer

提交于 Jan 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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