The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23300543
Use at an assay dependent concentration. PubMed: 27581371
Use at an assay dependent concentration. PubMed: 20889618
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Use a concentration of 10 - 20 µg/ml. PubMed: 19704022
1/100. PubMed: 19474334
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
GFP like chromoprotein antibody
red fluorescent protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab62341)This image is courtesy of an anonymous Abreview.
ab62341, at 1/100, staining chicken midbrain cells by Immunocytochemistry/ Immunofluorescence.The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody for 3 hours. An Alexa fluor® 568 goat polyclonal to rabbit Ig, diluted 1/300, was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded HT29 cells tissue labeling RFP with ab62341 at 1400 dilution,followed by Goat Anti-Rabbit (HRP) (ab62341)
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab62341)Image courtesy of an anonymous Abreview.
ab62341 staining RFP in rat oligodendrocytes transfected with recombinant human adenovirus type 5 expressing Red Fluorescent Protein (RFP) by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 5% serum for 10 minutes at 25°C. Samples were then incubated with ab62341 at a 1/300 dilution for 1 hour at 25°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L), used at a 1/500 dilution.