概述

  • 产品名称
  • 描述
    兔多克隆抗体to RFP
  • 特异性
    ab62341 recognizes RFP and has been shown to react with tdTomato.
  • 经测试应用
    适用于: IHC-P, IHC-FrFl, IHC-Fr, WB, IP, ICC/IFmore details
  • 免疫原

    Recombinant full length protein corresponding to RFP.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 23300543
IHC-FrFl Use at an assay dependent concentration. PubMed: 27581371
IHC-Fr Use at an assay dependent concentration. PubMed: 20889618
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use a concentration of 10 - 20 µg/ml. PubMed: 19704022
ICC/IF 1/100. PubMed: 19474334

靶标

  • 相关性
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • 别名
    • DsRed antibody
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • RFP antibody

Anti-RFP antibody 图像

  • ab62341, at 1/100, staining chicken midbrain cells by Immunocytochemistry/ Immunofluorescence.The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody for 3 hours. An Alexa fluor® 568 goat polyclonal to rabbit Ig, diluted 1/300, was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-RFP antibody (ab62341) at 1/2000 dilution

    Lane 1 : Whole cell lysate prepared from pDsRed-Monomer transfected HEK293 cells
    Lane 2 : Whole cell lysate prepared from pDsRed-Monomer transfected HEK293 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated anti-rabbit at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

  • Immunohistochemical analysis of paraffin-embedded HT29 cells tissue labeling RFP with ab62341 at 1400 dilution,followed by Goat Anti-Rabbit (HRP) (ab62341)

  • ab62341 staining RFP in rat oligodendrocytes transfected with recombinant human adenovirus type 5 expressing Red Fluorescent Protein (RFP) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with formaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 5% serum for 10 minutes at 25°C. Samples were then incubated with ab62341 at a 1/300 dilution for 1 hour at 25°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L), used at a 1/500 dilution.

    See Abreview

Anti-RFP antibody (ab62341)参考文献

This product has been referenced in:
  • Duclos CM  et al. Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling. Cell Death Discov 3:16100 (2017). Read more (PubMed: 28179995) »
  • Davey CF  et al. PCP Signaling between Migrating Neurons and their Planar-Polarized Neuroepithelial Environment Controls Filopodial Dynamics and Directional Migration. PLoS Genet 12:e1005934 (2016). IHC - Wholemount . Read more (PubMed: 26990447) »

See all 58 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Colon)
Specification
Colon
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 18 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (Mouse Epiblast stem cells expressing Dsred)
Specification
Mouse Epiblast stem cells expressing Dsred
Permeabilization
Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Fixative
Paraformaldehyde
Username

Miss. Yali Huang

Verified customer

提交于 Nov 29 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Sample
Rat Tissue sections (pituitary gland of mRFP1 gene transgenic rat)
Specification
pituitary gland of mRFP1 gene transgenic rat
Permeabilization
Yes - Triton-X
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 30 2013

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Monkey Cell lysate - other (cos7 cell)
Specification
cos7 cell
Treatment
overexpress RFP construct
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cell)
Specification
Hela cell
Permeabilization
No
Fixative
Formaldehyde
Username

Ms. Ya Zhou

Verified customer

提交于 Sep 19 2013

Thank you for contacting us.

This product should recognize dsRed as well as RFP, but will not bind to GFP or coGFP. It has not been tested against mCherry however the immunogen only shares a 74% identity with the mCherry sequence (GenBank:...

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (HT29 cells expressing DsRed protein)
Antigen retrieval step
Enzymatic
Permeabilization
Yes - Tween-20
Specification
HT29 cells expressing DsRed protein
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 28 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
56000 cells
Treatment
Transfeceted for 24 hours
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2015

Yes, this product detects the DS red monomer. The WB in the image was run using dsRed monomer protein with a His-tag.

1-10 of 77 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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