Anti-RFP抗体(ab152123)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RFP抗体
参阅全部 RFP 一抗 -
描述
兔多克隆抗体to RFP -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant full length RFP.
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阳性对照
- RFP-transfected HeLa cells; RFP-transfected 293T lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab152123于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 27 kDa.
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IP |
1/10000.
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ICC/IF |
1/5000.
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说明 |
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WB
1/5000 - 1/20000. Predicted molecular weight: 27 kDa. |
IP
1/10000. |
ICC/IF
1/5000. |
靶标
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相关性
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
别名
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
图片
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All lanes : Anti-RFP antibody (ab152123) at 1/10000 dilution
Lane 1 : Non-transfected 293T whole cell lysate
Lane 2 : GFP-transfected 293T whole cell lysate
Lane 3 : RFP-transfected 293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Immunoprecipitation of RFP-tagged protein.
HeLa transfected lysates were subjected to immunoprecipitation using:
Lane 1: 15 µg of HeLa transfected lysate
Lane 2: normal rabbit IgG
Lane 3: 2.5 µg ab152123
Detection ab152123 at 1/10000 dilution -
Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab152123 被引用在 3 文献中.
- Zhou Y et al. Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1. J Virol 95:e0015321 (2021). PubMed: 34287041
- Lu X et al. Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival via upregulating lncRNA 003946 in a rat model of corneal allograft rejection. Theranostics 10:8446-8467 (2020). PubMed: 32724480
- Joseph SR et al. Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos. Elife 6:N/A (2017). IP . PubMed: 28425915