We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Chimpanzee, Gorilla, Orangutan
Synthetic peptide (Human) - which represented a portion of human Replication Factor C 5 36 kDa subunit encoded within exon 1.
HeLa cell nuclear extract.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
Belongs to the activator 1 small subunits family.
Information by UniProt
36 kDa subunit antibody
A1 36 antibody
A1 36 kDa subunit antibody
Western blot - RFC5 antibody (ab3619)
Predicted band size :
µg Hela cell nuclear extract
Anti-RFC 5 (ab3619) used at 1:1000 dilution
This product has been referenced in:
et al. Replication factor C recruits DNA polymerase delta to sites of nucleotide excision repair but is not required for PCNA recruitment. Mol Cell Biol 30:4828-39 (2010).
Read more (PubMed: 20713449) »
et al. Expression of catalase mRNA and protein in adult rat brain: detection by nonradioactive in situ hybridization with signal amplification by catalyzed reporter deposition (ISH-CARD) and immunohistochemistry (IHC)/immunofluorescence (IF). J Histochem Cytochem 51:751-60 (2003).
Read more (PubMed: 12754286) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"