RFC4 overexpression 293T裂解液(全细胞) (ab94287)

概述

  • 产品名称RFC4 overexpression 293T裂解液(全细胞)
  • 描述
    RFC4 overexpression裂解液
  • 常规说明ab94287 is a 293T cell transfected lysate in which Human RFC4 has been transiently over-expressed using a pCMV-RFC4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Function: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. Similarity: Belongs to the activator 1 small subunits family.

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应用

Our Abpromise guarantee covers the use of ab94287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

RFC4 overexpression 293T lysate (whole cell) 图像

  • ab94287 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RFC4 antibody (ab57917) at 1/500 dilution

    Lane 1 : RFC4 overexpression 293T lysate (whole cell) (ab94287)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

RFC4 overexpression 293T lysate (whole cell) (ab94287)参考文献

ab94287 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"