Anti-RFC4抗体(ab96852)
Key features and details
- Rabbit polyclonal to RFC4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RFC4抗体
参阅全部 RFC4 一抗 -
描述
兔多克隆抗体to RFC4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 18 and 293 of Human RFC4 (NP_002907)
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阳性对照
- 293T, A431, H1299, HeLa, HepG, Molt-4, Raji cell lysates. A549 cells. MDAMB-468 Xenograft
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96852于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template. -
序列相似性
Belongs to the activator 1 small subunits family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 504637 Cow
- Entrez Gene: 5984 Human
- Entrez Gene: 106344 Mouse
- Entrez Gene: 288003 Rat
- Omim: 102577 Human
- SwissProt: P35249 Human
- SwissProt: Q99J62 Mouse
- Unigene: 714318 Human
see all -
别名
- A1 37 antibody
- A1 37 kDa subunit antibody
- Activator 1 37 antibody
see all
图片
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All lanes : Anti-RFC4 antibody (ab96852) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab96852 at 1/200 dilution. Lower image merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using ab96852 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab96852 被引用在 1 文献中.
- Yenerall P et al. RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Cell Chem Biol 27:105-121.e14 (2020). PubMed: 31883965