概述

  • 产品名称Anti-RFC1抗体
    参阅全部 RFC1 一抗
  • 描述
    兔多克隆抗体to RFC1
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1, 140 kDa subunit encoded in part by exons 25 and 26.

  • 阳性对照
    • Nuclear extract from HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 128 kDa).

靶标

  • 功能The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
    Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
  • 组织特异性Wide tissue distribution. Undetectable in placental tissue.
  • 序列相似性Belongs to the activator 1 large subunit family.
    Contains 1 BRCT domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1 140 kDa subunit antibody
    • A1 antibody
    • A1 P145 Activator 1 large subunit antibody
    • Activator 1 140 kDa subunit antibody
    • Activator 1 large subunit antibody
    • Activator 1 subunit 1 antibody
    • DNA binding Protein PO GA antibody
    • DNA-binding protein PO-GA antibody
    • MHC binding factor beta antibody
    • MHCBFB antibody
    • PO GA antibody
    • RECC1 antibody
    • Replication factor C (activator 1) 1, 145kDa antibody
    • Replication factor C 140 kDa subunit antibody
    • Replication factor C antibody
    • Replication factor C large subunit antibody
    • Replication factor C subunit 1 antibody
    • Replication factor C1 antibody
    • RF-C 140 kDa subunit antibody
    • RFC antibody
    • RFC1 antibody
    • RFC1_HUMAN antibody
    • RFC140 antibody
    • RFC140 Replication Factor C 140 kDa subunit antibody
    see all

Anti-RFC1 antibody 图像

  • Detection of Human RFC 1 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 1 (ab3853)

    Sample: Nuclear Extract (NE, amounts used are indicated) from HeLa cells.

    Antibody: Affinity Purified Rb anti-RFC 1 (ab3853).

    WB - 0.5µg/ml, IP - 2µg/mg NE.

    Detection: ECL, 30 seconds

     

Anti-RFC1 antibody (ab3853)参考文献

This product has been referenced in:
  • Lee KY  et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . Read more (PubMed: 23277426) »
  • Lee KY  et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). Read more (PubMed: 20147293) »

See all 2 Publications for this product

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