概述

  • 产品名称
  • 描述
    兔多克隆抗体to RFC1
  • 经测试应用
    适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1, 140 kDa subunit encoded in part by exons 25 and 26.

  • 阳性对照
    • Nuclear extract from HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 128 kDa).

靶标

  • 功能
    The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
    Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
  • 组织特异性
    Wide tissue distribution. Undetectable in placental tissue.
  • 序列相似性
    Belongs to the activator 1 large subunit family.
    Contains 1 BRCT domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1 140 kDa subunit antibody
    • A1 antibody
    • A1 P145 Activator 1 large subunit antibody
    • Activator 1 140 kDa subunit antibody
    • Activator 1 large subunit antibody
    • Activator 1 subunit 1 antibody
    • DNA binding Protein PO GA antibody
    • DNA-binding protein PO-GA antibody
    • MHC binding factor beta antibody
    • MHCBFB antibody
    • PO GA antibody
    • RECC1 antibody
    • Replication factor C (activator 1) 1, 145kDa antibody
    • Replication factor C 140 kDa subunit antibody
    • Replication factor C antibody
    • Replication factor C large subunit antibody
    • Replication factor C subunit 1 antibody
    • Replication factor C1 antibody
    • RF-C 140 kDa subunit antibody
    • RFC antibody
    • RFC1 antibody
    • RFC1_HUMAN antibody
    • RFC140 antibody
    • RFC140 Replication Factor C 140 kDa subunit antibody
    see all

Anti-RFC1 antibody 图像

  • Detection of Human RFC 1 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 1 (ab3853)

    Sample: Nuclear Extract (NE, amounts used are indicated) from HeLa cells.

    Antibody: Affinity Purified Rb anti-RFC 1 (ab3853).

    WB - 0.5µg/ml, IP - 2µg/mg NE.

    Detection: ECL, 30 seconds

     

Anti-RFC1 antibody (ab3853)参考文献

This product has been referenced in:
  • Lee KY  et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . Read more (PubMed: 23277426) »
  • Lee KY  et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). Read more (PubMed: 20147293) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab3853.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册