概述

  • 产品名称Anti-REV3L抗体
    参阅全部 REV3L 一抗
  • 描述
    小鼠单克隆抗体to REV3L
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fusion protein: LAGLESDGYR GERNRMPSPC RSFGNNKYPQ NSDDEENEPQ IEKEEMELSL VMSQRWDSNI EEHCAKKRSL CRNTHRSSTE DDDSSSGEEM EWSDNSLLLA , corresponding to amino acids 418-518 of Human REV3L

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆单克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 352 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性This Product recognizes the catalytic subunit of DNA polymerase zeta a nonessential enzyme that is thought to carry out translation replication and is responsible for virtually all DNA damage-induced mutagenesis and the majority of spontaneous mutagenesis.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Catalytic subunit of DNA polymerase zeta antibody
    • Polymerase DNA zeta antibody
    • POLZ antibody
    • RAD54 like antibody
    • REV3 yeast homolog like antibody
    • Sez4 antibody
    • Yeast homolog of Rev 3 antibody
    see all

Anti-REV3L antibody 图像

  • All lanes : Anti-REV3L antibody (ab52704) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100ng of a Tagged irrelevant antigen (Negative control)
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) HRP at 1/5000 dilution

    Predicted band size : 352 kDa

Anti-REV3L antibody (ab52704)参考文献

ab52704 has not yet been referenced specifically in any publications.

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