The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
This Product recognizes the catalytic subunit of DNA polymerase zeta a nonessential enzyme that is thought to carry out translation replication and is responsible for virtually all DNA damage-induced mutagenesis and the majority of spontaneous mutagenesis.