Anti-REV3L抗体(ab111729)

概述

  • 产品名称
    Anti-REV3L抗体
  • 描述
    兔多克隆抗体to REV3L
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from internal region of Human REV3L.

  • 阳性对照
    • Human brain tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab111729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    This Product recognizes the catalytic subunit of DNA polymerase zeta a nonessential enzyme that is thought to carry out translation replication and is responsible for virtually all DNA damage-induced mutagenesis and the majority of spontaneous mutagenesis.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • Catalytic subunit of DNA polymerase zeta antibody
    • Polymerase DNA zeta antibody
    • POLZ antibody
    • RAD54 like antibody
    • REV3 yeast homolog like antibody
    • Sez4 antibody
    • Yeast homolog of Rev 3 antibody
    see all

图片

  • ab111729 at 1/50 dilution staining REV3L in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

文献

ab111729 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Cell (HEK293 cell line)
Specification
HEK293 cell line
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde
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提交于 May 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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