Anti-REV1抗体(ab5088)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to REV1
  • 经测试应用
    适用于: ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    VLQQTYGSTLKVT

    , corresponding to amino acids 1239-1251 of Human REV 1L.

  • 常规说明


    REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明
    REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
  • 克隆
    多克隆
  • 同种型
    IgG2c
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 4 - 6 µg/ml.

靶标

  • 功能
    Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the DNA polymerase type-Y family.
    Contains 1 BRCT domain.
    Contains 1 umuC domain.
  • 结构域
    The C-terminal domain is necessary for protein interactions.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIBP 80 antibody
    • AIBP80 antibody
    • Alpha integrin binding protein 80 antibody
    • Alpha integrin-binding protein 80 antibody
    • DNA repair protein REV 1 antibody
    • DNA repair protein REV1 antibody
    • FLJ21523 antibody
    • MGC163283 antibody
    • MGC26225 antibody
    • REV 1 (yeast homolog) like antibody
    • REV 1 antibody
    • REV 1 homolog antibody
    • REV 1 like antibody
    • REV 1 protein antibody
    • REV 1L antibody
    • REV1 (yeast homolog) like antibody
    • REV1 antibody
    • REV1 homolog (S. cerevisiae) antibody
    • REV1 homolog antibody
    • REV1 like (yeast) antibody
    • REV1 like antibody
    • Rev1 like terminal deoxycytidyl transferase antibody
    • REV1 protein antibody
    • Rev1-like terminal deoxycytidyl transferase antibody
    • REV1_HUMAN antibody
    • REV1L antibody
    see all

Anti-REV1 antibody 图像

  • In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. ab5088 used at 4ug/ml following Steamed antigen retrieval with Tris/EDTA buffer pH 9 visualised using HRP staining.

Anti-REV1 antibody (ab5088)参考文献

ab5088 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP-40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
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提交于 May 16 2016

Thank you for your email. Ab5088 is a Fast-Track antibody and has not yet been fully characterized. We are unable to provide a discount, but we will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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