Retinoid X Receptor gamma overexpression 293T裂解液(全细胞) (ab94313)

概述

  • 产品名称Retinoid X Receptor gamma overexpression 293T裂解液(全细胞)
  • 常规说明ab94313 is a 293T cell transfected lysate in which Human Retinoid X Receptor gamma has been transiently over-expressed using a pCMV-Retinoid X Receptor gamma plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. Similarity: Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain. Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

应用

Our Abpromise guarantee covers the use of ab94313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Retinoid X Receptor gamma overexpression 293T lysate (whole cell) 图像

  • ab94313 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Retinoid X Receptor gamma antibody (ab57671) at 1/500 dilution

    Lane 1 : Retinoid X Receptor gamma overexpression 293T lysate (whole cell) (ab94313)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Retinoid X Receptor gamma overexpression 293T lysate (whole cell) (ab94313)参考文献

ab94313 has not yet been referenced specifically in any publications.

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