Anti-Retinoid X Receptor alpha抗体(ab86027)


  • 产品名称Anti-Retinoid X Receptor alpha抗体
    参阅全部 Retinoid X Receptor alpha 一抗
  • 描述
    兔多克隆抗体to Retinoid X Receptor alpha
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Three synthetic peptides (human) conjugated to KLH

  • 阳性对照
    • An induced culture of E. coli


Our Abpromise guarantee covers the use of ab86027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明WB: 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa). Tag is 14kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
    • 组织特异性Highly expressed in liver, also found in lung, kidney and heart.
    • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
    • 翻译后修饰Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
      Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ00280 antibody
      • FLJ00318 antibody
      • FLJ16020 antibody
      • FLJ16733 antibody
      • MGC102720 antibody
      • NR2B1 antibody
      • Nuclear receptor subfamily 2 group B member 1 antibody
      • OTTHUMP00000022510 antibody
      • Retinoic acid receptor RXR alpha antibody
      • Retinoic acid receptor RXR-alpha antibody
      • Retinoid X nuclear receptor alpha antibody
      • Retinoid X receptor alpha antibody
      • RXR alpha1 antibody
      • Rxra antibody
      • RXRA_HUMAN antibody
      • RXRalpha1 antibody
      see all

    Anti-Retinoid X Receptor alpha antibody 图像

    • All lanes : Anti-Retinoid X Receptor alpha antibody (ab86027) at 1/1000 dilution

      Lane 1 : An un-induced culture of E. coli
      Lane 2 : An induced culture of E. coli

      Lysates/proteins at 1/5000 dilution per lane.

      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 51 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa,46 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 60 minutes

    Anti-Retinoid X Receptor alpha antibody (ab86027)参考文献

    ab86027 has not yet been referenced specifically in any publications.

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