Anti-Retinoid X Receptor alpha抗体(ab80453)

概述

  • 产品名称Anti-Retinoid X Receptor alpha抗体
    参阅全部 Retinoid X Receptor alpha 一抗
  • 描述
    兔多克隆抗体to Retinoid X Receptor alpha
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow
  • 免疫原

    Synthetic peptide: SLGPGIGSPG QLHSPISTLS SPINGMGPPF SVISSPMGPH SMSVPTTPTL , corresponding to N terminal amino acids 35-84 of Human Retinoid X Receptor alpha (NP_002948)

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
  • 组织特异性Highly expressed in liver, also found in lung, kidney and heart.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
  • 翻译后修饰Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
    Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ00280 antibody
    • FLJ00318 antibody
    • FLJ16020 antibody
    • FLJ16733 antibody
    • MGC102720 antibody
    • NR2B1 antibody
    • Nuclear receptor subfamily 2 group B member 1 antibody
    • OTTHUMP00000022510 antibody
    • Retinoic acid receptor RXR alpha antibody
    • Retinoic acid receptor RXR-alpha antibody
    • Retinoid X nuclear receptor alpha antibody
    • Retinoid X receptor alpha antibody
    • RXR alpha1 antibody
    • Rxra antibody
    • RXRA_HUMAN antibody
    • RXRalpha1 antibody
    see all

Anti-Retinoid X Receptor alpha antibody 图像



  • Predicted band size : 51 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling Retionoid X Receptor alpha with ab80453 at 1.0µg/ml.


  • Predicted band size : 51 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling Retionoid X Receptor alpha with ab80453 at 1.0µg/ml.


  • Predicted band size : 51 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling Retionoid X Receptor alpha with ab80453 at 1.0µg/ml.


  • Predicted band size : 51 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling Retionoid X Receptor alpha with ab80453 at 1.0µg/ml.
  • Anti-Retinoid X Receptor alpha antibody (ab80453) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

Anti-Retinoid X Receptor alpha antibody (ab80453)参考文献

ab80453 has not yet been referenced specifically in any publications.

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