Anti-Retinoid X Receptor alpha/RXRA抗体(ab227273)
Key features and details
- Rabbit polyclonal to Retinoid X Receptor alpha/RXRA
- Suitable for: WB, IP
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Retinoid X Receptor alpha/RXRA抗体
参阅全部 Retinoid X Receptor alpha/RXRA 一抗 -
描述
兔多克隆抗体to Retinoid X Receptor alpha/RXRA -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Cow -
免疫原
Recombinant fragment within Human Retinoid X Receptor alpha/RXRA (internal sequence). The exact sequence is proprietary.
Database link: P19793 -
阳性对照
- WB: Rat liver tissue lysate; HepG2 whole cell lysate. IP: MCF7 whole cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227273于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/10000. Predicted molecular weight: 51 kDa.
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IP |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/10000. Predicted molecular weight: 51 kDa. |
IP
1/100 - 1/500. |
靶标
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功能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. -
组织特异性
Highly expressed in liver, also found in lung, kidney and heart. -
序列相似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain). -
翻译后修饰
Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6256 Human
- Entrez Gene: 20181 Mouse
- Entrez Gene: 25271 Rat
- Omim: 180245 Human
- SwissProt: P19793 Human
- SwissProt: P28700 Mouse
- SwissProt: Q05343 Rat
- Unigene: 590886 Human
see all -
别名
- FLJ00280 antibody
- FLJ00318 antibody
- FLJ16020 antibody
see all
图片
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Anti-Retinoid X Receptor alpha/RXRA antibody (ab227273) at 1/500 dilution + Rat liver tissue lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Retinoid X Receptor alpha/RXRA was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 4 μg ab227273. Western blot was performed from the immunoprecipitate using ab227273 at 1/500 dilution.
Lane 1: Control IgG IP in MCF7 whole cell extract.
Lane 2: ab227273 IP in MCF7 whole cell extract.
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Anti-Retinoid X Receptor alpha/RXRA antibody (ab227273) at 1/5000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227273 尚未被引用在任何文献中。