RabMAb

Anti-Retinoic Acid Receptor beta抗体[EPR2017] (ab124701)

概述

  • 产品名称Anti-Retinoic Acid Receptor beta抗体[EPR2017]
    参阅全部 Retinoic Acid Receptor beta 一抗
  • 描述
    兔单克隆抗体[EPR2017] to Retinoic Acid Receptor beta
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Retinoic Acid Receptor beta aa 400 to the C-terminus.

  • 阳性对照
    • Human kidney tissue; MCF7, MDA-MB-435, Daudi and U-87 MG cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Retinoic Acid Receptor beta antibody (HRP) [EPR2017] (ab198557)

性能

应用

Our Abpromise guarantee covers the use of ab124701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
    • 疾病相关Microphthalmia, syndromic, 12
    • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 细胞定位Cytoplasm and Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HAP antibody
      • HBV-activated protein antibody
      • NR1B2 antibody
      • Nuclear receptor subfamily 1 group B member 2 antibody
      • RAR B antibody
      • RAR beta antibody
      • RAR epsilon antibody
      • RAR-beta antibody
      • RAR-epsilon antibody
      • RARB antibody
      • RARB_HUMAN antibody
      • Retinoic acid receptor beta 2 antibody
      • Retinoic acid receptor beta 4 antibody
      • Retinoic acid receptor beta 5 antibody
      • Retinoic acid receptor beta antibody
      • Retinoic acid receptor beta polypeptide antibody
      • RRB2 antibody
      see all

    Anti-Retinoic Acid Receptor beta antibody [EPR2017] 图像

    • All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/1000 dilution

      Lane 1 : MCF7 cell lysates
      Lane 2 : MDA-MB-435 cell lysates
      Lane 3 : Daudi cell lysates
      Lane 4 : U-87 MG cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lane 1 : Goat anti-Rabbit HRP at 1/20000 dilution
      Lanes 2 - 4 : Goat anti-Rabbit HRP at 1/2000 dilution


      Predicted band size : 50 kDa
    • ab124701 at 1/100 dilution staining Retinoic Acid Receptor beta in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701)参考文献

    ab124701 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Adult Testis)
    Specification Adult Testis
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization No
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    煜欽 林

    Verified customer

    提交于 Jan 17 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Marmoset (common) Tissue sections (Adult testis)
    Specification Adult testis
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization No
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    煜欽 林

    Verified customer

    提交于 Jan 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"