Anti-Retinoic Acid Receptor beta抗体(ab76778)

概述

  • 产品名称
    Anti-Retinoic Acid Receptor beta抗体
    参阅全部 Retinoic Acid Receptor beta 一抗
  • 描述
    小鼠多克隆抗体to Retinoic Acid Receptor beta
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-448 of Human Retinoic Acid Receptor beta

  • 阳性对照
    • HeLa cells RARB transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab76778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • 疾病相关
    Microphthalmia, syndromic, 12
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位
    Cytoplasm and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HAP antibody
    • HBV-activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB2 antibody
    see all

图片

  • All lanes : Anti-Retinoic Acid Receptor beta antibody (ab76778) at 1/500 dilution

    Lane 1 : Retinoic Acid Receptor beta transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • HeLa cells labelled with ab76778 at 10 µg/ml followed by fluorescein conjugated secondary antibody.

文献

ab76778 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

提交于 Mar 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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