Anti-Retinoic Acid Receptor beta抗体(ab53161)

概述

  • 产品名称
    Anti-Retinoic Acid Receptor beta抗体
    参阅全部 Retinoic Acid Receptor beta 一抗
  • 描述
    兔多克隆抗体to Retinoic Acid Receptor beta
  • 经测试应用
    适用于: WB, ELISA, IHC-P, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta

  • 阳性对照
    • Human breast carcinoma tissue HepG2 cell extract

性能

应用

Our Abpromise guarantee covers the use of ab53161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20655472
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • 疾病相关
    Microphthalmia, syndromic, 12
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位
    Cytoplasm and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HAP antibody
    • HBV-activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB2 antibody
    see all

图片

  • This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
  • All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilution

    Lane 1 : HepG2 cell extract, untreated.
    Lane 2 : HepG2 cell extract treated with the immunising peptide.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rat . Read more (PubMed: 28190929) »
  • Doldo E  et al. High expression of cellular retinol binding protein-1 in lung adenocarcinoma is associated with poor prognosis. Genes Cancer 6:490-502 (2015). IHC . Read more (PubMed: 26807202) »

See all 7 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult testis)
Specification
adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

提交于 Jan 18 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (marmoset adult testis section)
Specification
marmoset adult testis section
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

提交于 Jan 18 2013

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab53161 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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