Anti-Retinoic Acid Receptor beta抗体(ab15515)

概述

  • 产品名称Anti-Retinoic Acid Receptor beta抗体
    参阅全部 Retinoic Acid Receptor beta 一抗
  • 描述
    兔多克隆抗体to Retinoic Acid Receptor beta
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • 阳性对照
    • Testis and heart.

性能

应用

Our Abpromise guarantee covers the use of ab15515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • 疾病相关Microphthalmia, syndromic, 12
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Cytoplasm and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HAP antibody
    • HBV-activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB2 antibody
    see all

Anti-Retinoic Acid Receptor beta antibody 图像

  • ab15515 staining Reinoic Acid Receptor beta at 1/100 dilution in human testis by Immunohistochemistry (FFPE-sections).
  • ab15515 at 1/100 staining hamster CHO-K1 cells expressing human retinoic acid receptor by ICC/IF. The cells were formaldehyde fixed and blocked before incubation with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Anti-Retinoic Acid Receptor beta antibody (ab15515) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

Anti-Retinoic Acid Receptor beta antibody (ab15515)参考文献

This product has been referenced in:
  • Childs AJ  et al. Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. PLoS One 6:e20249 (2011). IHC-P ; Human . Read more (PubMed: 21674038) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO-K1 cells expressing Human Retinoic Acid Recept)
Specification CHO-K1 cells expressing Human Retinoic Acid Recept
Fixative Formaldehyde
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

提交于 Aug 21 2007

To our knowledge, this antibody has not yet been tested in WB.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"