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Our Abpromise guarantee covers the use of ab15515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).|
ab15515 at 1/100 staining hamster CHO-K1 cells expressing human retinoic acid receptor by ICC/IF. The cells were formaldehyde fixed and blocked before incubation with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.