Anti-Retinoic Acid Receptor alpha抗体(ab28767)

概述

  • 产品名称Anti-Retinoic Acid Receptor alpha抗体
    参阅全部 Retinoic Acid Receptor alpha 一抗
  • 描述
    山羊多克隆抗体to Retinoic Acid Receptor alpha
  • 特异性This antibody is expected to recognize all three reported [human] isoforms (NP_000955.1; NP_001019980.1; NP_001028775.1).
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Hamster, Cow, Dog
  • 免疫原

    Synthetic peptide:

    CSPSLSPSSH RSSPATQSP

    , corresponding to C terminal amino acids 441-459 of Retinoic Acid Receptor alpha

  • 阳性对照
    • Human brain lysate. Human cerebellum tissue sections.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab28767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50. See Abreview.
WB Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 51 kDa).Can be blocked with Retinoic Acid Receptor alpha peptide (ab4910).
IHC-P Use a concentration of 4 - 6 µg/ml. Steamed antigen retrieval is recommended using citrate buffer pH 6.0.

靶标

  • 功能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis.
  • 疾病相关Note=Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosporylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.
    Sumoylated by SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.
    Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.
    Ubiquitinated.
  • 细胞定位Nucleus. Cytoplasm. Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NR1B1 antibody
    • Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody
    • Nuclear receptor subfamily 1 group B member 1 antibody
    • Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibody
    • RAR alpha antibody
    • RAR antibody
    • RAR-alpha antibody
    • rara antibody
    • RARA_HUMAN antibody
    • RARalpha antibody
    • RARalpha1 antibody
    • Retinoic acid nuclear receptor alpha variant 1 antibody
    • Retinoic acid nuclear receptor alpha variant 2 antibody
    • Retinoic acid receptor alpha antibody
    • Retinoic acid receptor alpha polypeptide antibody
    see all

Anti-Retinoic Acid Receptor alpha antibody 图像

  • Anti-Retinoic Acid Receptor alpha antibody (ab28767) at 0.03 µg/ml + human brain lysate (30µg total protein per lane)
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 48,50 kDa (why is the actual band size different from the predicted?)
  • ab28767 at 4µg/ml staining Retinoic Acid Receptor alpha in human cerebellum tissue. Steamed antigen retrieval using citrate buffer pH6 was performed. AP staining. Image shows staining of Purkinje cytoplasm.

Anti-Retinoic Acid Receptor alpha antibody (ab28767)参考文献

This product has been referenced in:
  • Joss-Moore L  et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). Rat . Read more (PubMed: 23419538) »
  • Mengeling BJ  et al. Aberrant Corepressor Interactions Implicated in PML-RAR{alpha} and PLZF-RAR{alpha} Leukemogenesis Reflect an Altered Recruitment and Release of Specific NCoR and SMRT Splice Variants. J Biol Chem 286:4236-47 (2011). WB . Read more (PubMed: 21131350) »

See all 5 Publications for this product

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Again let me emphasize that I am sorry this product is not performing. These complaints are taken seroiusly and this will be looked into by the appropriate groups. I am sorry that the product did not work against...

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Thank you for contact me with this further information. I agree that the positvie control looks very poor, however I am hopeful now as we can use that control to clean up the signal. I would recommend using a 5% milk blocking solution for at least 1...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for providing these additional details and images.

I would like to send you the postive control for these products. Using the positve control will provide valuable data in this case as having two products against the target both no...

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Thank you for contacting Abcam.

I would like to thank you for supplying information about your protocol and I am sorry to hear that ab28767 has not been performing for you. I am most concerned that you found no difference in your blots after...

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Thank you for contacting us. Your credit note ID are: Order No: 896459 - Credit note: 188XX Order No: 944015 - Credit note: 188XX Order No: 955405 - Credit note: 188XX I am sorry that this antibody did not perform as stated on the ...

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Thank you for your response. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a ...

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Thank you for getting back to me and resending the WB images. It looks that the major band(s) are around 40 kDa slightly lower than the expected 50 kDa. These lower bands could either represent modified (processed Retinoic Acid Receptor alpha) or...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am reviewing your enquiries but unfortunately without knowing what exactly MW markers represent it is difficult to assess the ban...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with these two products (ab28767 and ab76074). I appreciate the time you...

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