The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 45 kDa.
Use at an assay dependent concentration.
功能Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase.
组织特异性Retina and pineal gland.
疾病相关Defects in SAG are a cause of congenital stationary night blindness Oguchi type 1 (CSNBO1) [MIM:258100]; also known as Oguchi disease. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. CSNBO is an autosomal recessive form associated with fundus discoloration and abnormally slow dark adaptation.
Immunohistochemistry was performed on Human retina tissue. To expose target protein antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Retinal S antigen mouse monoclonal antibody (ab111108) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
Western blot - Retinal S antigen antibody [PDS1] (ab111108)
Anti-Retinal S antigen antibody [PDS1] (ab111108) at 1/1000 dilution + Rat retina lysate
Predicted band size : 45 kDa
Anti-Retinal S antigen antibody [PDS1] (ab111108)参考文献
has not yet been referenced specifically in any publications.