Anti-Retinal S antigen抗体[PDS-1] (ab48360)

概述

  • 产品名称Anti-Retinal S antigen抗体[PDS-1]
    参阅全部 Retinal S antigen 一抗
  • 描述
    小鼠单克隆抗体[PDS-1] to Retinal S antigen
  • 特异性This antibody is specific for S antigen protein
  • 经测试应用适用于: Other, WB, IHC-Fr, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Rat, Cow, Human, Pig
  • 免疫原

    Porcine retinal S-antigen (arrestin).

  • 阳性对照
    • Retina lysate.

性能

应用

Our Abpromise guarantee covers the use of ab48360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
AP
Other
WB
IHC-Fr
ICC/IF
ICC
  • 应用说明AP (immunopurification of arrestin): Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-Fr: 1/100.
    WB: Use at an assay dependent dilution. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase.
    • 组织特异性Retina and pineal gland.
    • 疾病相关Defects in SAG are a cause of congenital stationary night blindness Oguchi type 1 (CSNBO1) [MIM:258100]; also known as Oguchi disease. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. CSNBO is an autosomal recessive form associated with fundus discoloration and abnormally slow dark adaptation.
    • 序列相似性Belongs to the arrestin family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 48 kDa protein antibody
      • Arrestin 1 antibody
      • Arrestin antibody
      • ARRS_HUMAN antibody
      • Retinal S antigen (48 KDa protein) antibody
      • Retinal S-antigen antibody
      • Rod photoreceptor arrestin antibody
      • RP47 antibody
      • S antigen antibody
      • S antigen retina and pineal gland (arrestin) antibody
      • S arrestin antibody
      • S-AG antibody
      • S-arrestin antibody
      • SAG antibody
      see all

    Anti-Retinal S antigen antibody [PDS-1] 图像

    • ab43860 staining Retinal S antigen in cultured WERI-RB27 Human Retinoblastoma Cells by Immunocytochemistry. Cells were fixed in cytospin solution and permeabilized in Tween-PBS prior to blocking in 2.5% serum for 15 minutes at 21°C. The primary antibody was diluted 1/200 in PBS and incubated with the sample for 1 hour at 21°C. The secondary antibody was Biotin-conjugated Goat anti-Mouse polyclonal.

      See Abreview

    Anti-Retinal S antigen antibody [PDS-1] (ab48360)参考文献

    This product has been referenced in:
    • Banga JP  et al. Immunoaffinity purification of S-antigen using monoclonal antibodies to different antigenic sites. Invest Ophthalmol Vis Sci 28:604-7 (1987). Read more (PubMed: 3549614) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cultured Cells (WERI-RB27 Human Retinoblastoma Cells)
    Specification WERI-RB27 Human Retinoblastoma Cells
    Fixative Cytospin solution
    Permeabilization Yes - Tween-PBS
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 2.5% · Temperature: 21°C
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    提交于 Apr 01 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"