概述

  • 产品名称
    Anti-Reticulon 1A抗体[MON160]
    参阅全部 Reticulon 1A 一抗
  • 描述
    小鼠单克隆抗体[MON160] to Reticulon 1A
  • 特异性
    This antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues
  • 经测试应用
    适用于: IHC-P, Flow Cyt, ICC, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human
  • 免疫原

    Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

  • 表位
    This antibody is reactive with residues 174-337 of Reticulon-1A.
  • 常规说明


    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
  • 克隆
    单克隆
  • 克隆编号
    MON160
  • 骨髓瘤
    unknown
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.

Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/50 - 1/500.

靶标

  • 相关性
    Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.
  • 细胞定位
    Endoplasmic reticulum
  • 数据库链接
  • 别名
    • Neuroendocrine specific protein antibody
    • NSP antibody
    • Reticulon1 antibody
    • RTN1 antibody

图片

  • NSP-A immunostaining in human cerebellum using ab8957.

  • Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.

文献

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