概述

  • 产品名称Anti-Ret抗体[RET01]
    参阅全部 Ret 一抗
  • 描述
    小鼠单克隆抗体[RET01] to Ret
  • 特异性We do not know which isoform of Ret (Ret9 or Ret51) the antibody recognises.
  • 经测试应用适用于: IHC-P, IHC-Fr, ICC/IF, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Human
  • 免疫原

    BALB/C mice were injected with recombinant protein encoding the extreme C-terminal cytoplasmic region of the human ret oncoprotein.

  • 阳性对照
    • Small intestine
  • 常规说明


    Ret proto-oncogene encodes a cell surface glycoprotein belonging to a member of the receptor tyrosine kinase family and is located on chromosome 10q11.2. Ret is observed in several regions of the central nervous system; in the developing cranial nerve ganglia and a subset of cells within dorsal root ganglia, in motor neurons in the spinal cord and hindbrain.

性能

应用

Our Abpromise guarantee covers the use of ab1840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/10 - 1/20. ABC method.
ICC/IF 1/1. Use Neat
IHC (PFA fixed) 1/10.

靶标

  • 功能Probable receptor with tyrosine-protein kinase activity; important for development.
  • 疾病相关Defects in RET may be a cause of colorectal cancer (CRC) [MIM:114500].
    Defects in RET are a cause of Hirschsprung disease (HSCR) [MIM:142623]. HSCR is a genetic disorder of neural crest development characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Occasionally, MEN2A or FMTC occur in association with HSCR.
    Defects in RET are the cause of medullary thyroid carcinoma (MTC) [MIM:155240]. MTC is a rare tumor derived from the C cells of the thyroid. Three hereditary forms are known, that are transmitted in an autosomal dominant fashion: (a) multiple neoplasia type 2A (MEN2A), (b) multiple neoplasia type IIB (MEN2B) and (c) familial MTC (FMTC), which occurs in 25-30% of MTC cases and where MTC is the only clinical manifestation.
    Defects in RET are the cause of multiple neoplasia type 2B (MEN2B) [MIM:162300]. MEN2B is an uncommon inherited cancer syndrome characterized by predisposition to MTC and phaeochromocytoma which is associated with marfanoid habitus, mucosal neuromas, skeletal and ophtalmic abnormalities, and ganglioneuromas of the intestine tract. Then the disease progresses rapidly with the development of metastatic MTC and a pheochromocytome in 50% of cases.
    Defects in RET are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
    Defects in RET are the cause of multiple neoplasia type 2A (MEN2A) [MIM:171400]; also known as multiple neoplasia type 2 (MEN2). MEN2A is the most frequent form of medullary thyroid cancer (MTC). It is an inherited cancer syndrome characterized by MTC, phaeochromocytoma and/or hyperparathyroidism.
    Defects in RET are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving RET are found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q21) generates the RET/CCDC6 (PTC1) oncogene; inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene; translocation t(10;14)(q11;q32) with GOLGA5 generates the RET/GOLGA5 (PTC5) oncogene; translocation t(8;10)(p21.3;q11.2) with PCM1 generates the PCM1/RET fusion; translocation t(6;10)(p21.3;q11.2) with RFP generates the Delta RFP/RET oncogene; translocation t(1;10)(p13;q11) with TRIM33 generates the TRIM33/RET (PTC7) oncogene; translocation t(7;10)(q32;q11) with TRIM24/TIF1 generates the TRIM24/RET (PTC6) oncogene. The PTC5 oncogene has been found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes.
    Defects in RET are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.
    Defects in RET are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 cadherin domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C ret antibody
    • Cadherin family member 12 antibody
    • Cadherin related family member 16 antibody
    • CDHF 12 antibody
    • CDHF12 antibody
    • CDHR16 antibody
    • ELKS Fusion gene antibody
    • HSCR 1 antibody
    • HSCR1 antibody
    • Hydroxyaryl protein kinase antibody
    • MEN2A antibody
    • MEN2B antibody
    • MTC 1 antibody
    • MTC1 antibody
    • Multiple endocrine neoplasia and medullary thyroid carcinoma 1 antibody
    • Oncogene RET antibody
    • Proto oncogene tyrosine protein kinase receptor ret antibody
    • Proto-oncogene c-Ret antibody
    • Proto-oncogene tyrosine-protein kinase receptor ret antibody
    • PTC antibody
    • RET antibody
    • RET ELE1 antibody
    • Ret Proto oncogene antibody
    • RET transforming sequence antibody
    • RET_HUMAN antibody
    • RET51 antibody
    • RET9 antibody
    • tyrosine-protein kinase receptor ret antibody
    see all

Anti-Ret antibody [RET01] 图像

  • ab1840 - immunohistochemistry
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human intestine tissue, staining Ret with ab1840. Staining was detected using DAB.
  • ICC/IF image of ab1840 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1840, used neat (Undiluted)) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ret antibody [RET01] (ab1840)参考文献

This product has been referenced in:
  • Flowerdew SE  et al. Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection. PLoS One 8:e83603 (2013). IHC-P ; Human . Read more (PubMed: 24367603) »
  • Serra MP  et al. Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and fascia dentata. Int J Dev Neurosci 23:425-38 (2005). Read more (PubMed: 16002253) »

See all 2 Publications for this product

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We recommend to incubate primary antibodies at 4C over night for a standard IHC protocol. This will ensure the most specific binding and the best saturation with the antibody.

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ab1840:

Which serum is used as blocking agent is dependent on the secondary antibody that is used. The serum should be from the same species in which the secondary antibody was produced.

For exa...

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We usually use our standard protocol with our antibodies and do not perform optimization experiments. We usually use Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA Solution, 0.05% Tween 20, pH 9.0) and the microwave m...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (small intestine, ganglion cells)
Specification small intestine, ganglion cells
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, 100C, 20 minutes
Permeabilization No
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提交于 Oct 22 2009

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