重组
RabMAb

Anti-REST / NRSF抗体[EPR2436Y] (ab75785)

概述

  • 产品名称
    Anti-REST / NRSF抗体[EPR2436Y]
    参阅全部 REST / NRSF 一抗
  • 描述
    兔单克隆抗体[EPR2436Y] to REST / NRSF
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human REST/ NRSF

  • 阳性对照
    • Raji and HeLa cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab75785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 132 kDa.Can be blocked with REST / NRSF peptide (ab202336).
  • 应用说明
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能
      Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
    • 组织特异性
      Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
    • 序列相似性
      Contains 9 C2H2-type zinc fingers.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Neural Restrictive Silencer Factor antibody
      • Neural-restrictive silencer factor antibody
      • Neuron Restrictive Silencer Factor antibody
      • NRSF antibody
      • RE1 silencing transcription factor antibody
      • RE1-silencing transcription factor antibody
      • Repressor binding to the X2 box antibody
      • rest antibody
      • REST protein antibody
      • REST_HUMAN antibody
      • X2 box repressor antibody
      • XBR antibody
      see all

    图片

    • All lanes : Anti-REST / NRSF antibody [EPR2436Y] (ab75785) at 1/2000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 132 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    This product has been referenced in:
    • Bire S  et al. Mariner Transposons Contain a Silencer: Possible Role of the Polycomb Repressive Complex 2. PLoS Genet 12:e1005902 (2016). Read more (PubMed: 26939020) »
    • Ma P  et al. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A 109:E481-9 (2012). WB ; Mouse . Read more (PubMed: 22223663) »

    See all 2 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    90 µg
    Gel Running Conditions
    Reduced Denaturing (4-18% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification
    mouse embryonic stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 17 2015

    Thank you for contacting us.

    Any general Flow Cytometry protocol would work for this antibody. Please check the protocol below for guideline;

    http://www.epitomics.com/products/support/fc

    I hope this information is helpful to ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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