Anti-REST / NRSF抗体(ab21635)

概述

  • 产品名称Anti-REST / NRSF抗体
    参阅全部 REST / NRSF 一抗
  • 描述
    兔多克隆抗体to REST / NRSF
  • 经测试应用适用于: IHC-FoFr, WBmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Mouse REST/ NRSF.

    (Peptide available as ab17042.)

  • 阳性对照
    • This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab21635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 21715642Fixed for 10 min in cold methanol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
    • 组织特异性Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
    • 序列相似性Contains 9 C2H2-type zinc fingers.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Neural Restrictive Silencer Factor antibody
      • Neural-restrictive silencer factor antibody
      • Neuron Restrictive Silencer Factor antibody
      • NRSF antibody
      • RE1 silencing transcription factor antibody
      • RE1-silencing transcription factor antibody
      • Repressor binding to the X2 box antibody
      • rest antibody
      • REST protein antibody
      • REST_HUMAN antibody
      • X2 box repressor antibody
      • XBR antibody
      see all

    Anti-REST / NRSF antibody 图像

    • All lanes : Anti-REST / NRSF antibody (ab21635) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/ml
      Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 121 kDa
      Observed band size : 121 kDa
      Additional bands at : 53 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    Anti-REST / NRSF antibody (ab21635)参考文献

    This product has been referenced in:
    • Cox EJ & Marsh SA Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart. Cardiovasc Diabetol 12:101 (2013). Read more (PubMed: 23835259) »
    • Mapp OM  et al. Zebrafish Prickle1b mediates facial branchiomotor neuron migration via a farnesylation-dependent nuclear activity. Development 138:2121-32 (2011). IHC (PFA fixed) ; Zebrafish . Read more (PubMed: 21521740) »

    See all 7 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - nuclear (Hippocampus, liver and MCF7 whole cell homogenates)
    Gel Running Conditions Reduced Denaturing (10)
    Loading amount 80 µg
    Specification Hippocampus, liver and MCF7 whole cell homogenates
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Octavio Mercado-Gomez

    Verified customer

    提交于 May 12 2016

    Application Western blot
    Loading amount 90 µg
    Gel Running Conditions Reduced Denaturing (4-18% gel)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Specification Mouse embryonic stem cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 17 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization Yes - 0.3% TRITON-X
    Specification Brain
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2014

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case f...

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    Thank you for contacting us. I have forwarded your data to our lab for analysis. It would be very helpful if you could please provide some additional details about your protocol: 1.) How were the tissues fixed and processed (paraffin or froze...

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